Queen Melody (メロディ女王, Merodi joō) is the rightful ruler of Wonderland from TwinBee Yahoo!. Presumably she rules with a tolerant and just hand. Unfortunately, she gets deposed by Archduke Nonsense and his cronies, and even betrayed by her paramour, Ace. Fortunately, she has the good sense to call on TwinBee for help, so everything gets sorted out. Melody, like all of her subjects (except for Ace) has rather large ears. Her Kingdom has much in common with Lewis Carroll's Wonderland, being a surreal paradise filled with Cheshire Cats, playing card armies and that sort of thing. Queen Melody is voiced by Yuri Amano.

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